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proteinasek  (New England Biolabs)


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    Structured Review

    New England Biolabs proteinasek
    Proteinasek, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2790 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteinasek/Proteinase+K%2C+Molecular+Biology+Grade/pm41997155-664-17-18
    Average 99 stars, based on 2790 article reviews
    proteinasek - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Virus:

    Article Title: A chimeric vaccine derived from Australian genotype IV Japanese encephalitis virus protects mice from lethal challenge.
    Article Snippet: .. The RNA was extracted using the Macherey-Nagal Nucleospin RNA Virus kit as per the manufacturer’s instructions, with the following modifications: carrier RNA was omitted from the lysis buffer79; 20 μLof ProteinaseK (20mg/mL)was added to the initial lysis mixture and incubated at 70 °C for 5min; and an incolumn DNAse I disgestion step was performed after the first wash step by adding 5 μL DNAse I (NEB). .. The purified RNA was sequenced at the Australia Genome Research Facility (AGRF, Melbourne, Australia) using the NovaSeq SP Illumina platform, generating 2 ×150-base paired reads.

    Lysis:

    Article Title: A chimeric vaccine derived from Australian genotype IV Japanese encephalitis virus protects mice from lethal challenge.
    Article Snippet: .. The RNA was extracted using the Macherey-Nagal Nucleospin RNA Virus kit as per the manufacturer’s instructions, with the following modifications: carrier RNA was omitted from the lysis buffer79; 20 μLof ProteinaseK (20mg/mL)was added to the initial lysis mixture and incubated at 70 °C for 5min; and an incolumn DNAse I disgestion step was performed after the first wash step by adding 5 μL DNAse I (NEB). .. The purified RNA was sequenced at the Australia Genome Research Facility (AGRF, Melbourne, Australia) using the NovaSeq SP Illumina platform, generating 2 ×150-base paired reads.

    Incubation:

    Article Title: A chimeric vaccine derived from Australian genotype IV Japanese encephalitis virus protects mice from lethal challenge.
    Article Snippet: .. The RNA was extracted using the Macherey-Nagal Nucleospin RNA Virus kit as per the manufacturer’s instructions, with the following modifications: carrier RNA was omitted from the lysis buffer79; 20 μLof ProteinaseK (20mg/mL)was added to the initial lysis mixture and incubated at 70 °C for 5min; and an incolumn DNAse I disgestion step was performed after the first wash step by adding 5 μL DNAse I (NEB). .. The purified RNA was sequenced at the Australia Genome Research Facility (AGRF, Melbourne, Australia) using the NovaSeq SP Illumina platform, generating 2 ×150-base paired reads.

    Article Title: The discovery of 5mC-selective deaminases and their application to ultra-sensitive direct sequencing of methylated sites at base resolution
    Article Snippet: Reactions were cleaned with Zymo Oligo clean and concentrate columns, and eluted, then were digested with EcoRI-HF (NEB #R3101) for 1hr at 37°C in rCustmart® buffer (NEB #B6004S) following manufacturer recommendations. .. Reactions were stopped by the addition of 1μl of ProteinaseK (NEB #P8107S) and incubated at room temperature for 15 minutes. .. Finally oligos were diluted to 2nM and submitted for Capillary Electrophoresis on Applied Biosystems 3730xl DNA Analyzer machine running POP6 polymer.

    Article Title: Acute multi-level response to defective de novo chromatin assembly in S-phase
    Article Snippet: .. ProteinaseK (NEB) was added to the cell suspension to a final concentration of 75μg/ml followed by O.N. incubation on the shaking heat block at 55 C° 1000 rpm. ..

    Article Title: Shuttle peptide delivers base editor RNPs to rhesus monkey airway epithelial cells in vivo.
    Article Snippet: .. Following 1 h of incubation at 37 °C, ProteinaseK (NEB; catalog # P8107S)was diluted 4-fold in water and 5 μL of the diluted mixture was added to the cleavage reaction and incubated for 15min at 37 °C. ..

    Article Title: Acute multi-level response to defective de novo chromatin assembly in S-phase.
    Article Snippet: .. ProteinaseK (NEB) was added to the cell suspension to a final concentration of 75 mg/ml followed by O.N. incubation on the shaking heat block at 55 C 1000 rpm. ..

    Article Title: The discovery of 5mC-selective deaminases and their application to ultra-sensitive direct sequencing of methylated sites at base resolution.
    Article Snippet: Reactions were cleaned with Zymo Oligo clean and concentrate columns, and eluted, then were digested with EcoRIHF (NEB #R3101) for 1hr at 37 ◦ C in rCustmart® buffer (NEB #B6004S) following manufacturer recommendations. .. Reactions were e6 Molecular Cell 86, 1–16.e1–e11, May 7, 2026 stopped by the addition of 1μl of ProteinaseK (NEB #P8107S) and incubated at room temperature for 15 minutes. .. 9 For the same purpose of enriching cytosine modified phages in the microbiome, we conducted a second screen using treatment with a restriction enzyme (RE) cocktail containing HpaII, HaeIII, AluI, AciI, BfaI, HhaI, HpyCH4IV, HpyCH4V and NlaIII (all supplied by NEB).

    Concentration Assay:

    Article Title: Exploring roles of the chitinase ChiC in modulating Pseudomonas aeruginosa virulence phenotypes
    Article Snippet: .. A sample of 0.5 mL of a 1% casein solution (adjusted to pH 7.5) was combined with buffer and enzyme to a concentration of 25 mM Tris pH 7.5 and 1 μM ChiC, or 2 mg/mL ProteinaseK (NEB), respectively. .. The samples were incubated at 37°C with shaking (800 rpm) overnight (approximately 20 h) in a Thermomixer C (Eppendorf, Hamburg, Germany).

    Article Title: Acute multi-level response to defective de novo chromatin assembly in S-phase
    Article Snippet: .. ProteinaseK (NEB) was added to the cell suspension to a final concentration of 75μg/ml followed by O.N. incubation on the shaking heat block at 55 C° 1000 rpm. ..

    Article Title: Exploring roles of the chitinase ChiC in modulating Pseudomonas aeruginosa virulence phenotypes.
    Article Snippet: .. A sample of 0.5 mL of a 1% casein solution (adjusted to pH 7.5) was combined with buffer and enzyme to a concentration of 25 mM Tris pH 7.5 and 1 μM ChiC, or 2 mg/mL ProteinaseK (NEB), respectively. .. The samples were incubated at 37°C with shaking (800 rpm) overnight (approximately 20 h) in a Thermomixer C (Eppendorf, Hamburg, Germany).

    Article Title: Acute multi-level response to defective de novo chromatin assembly in S-phase.
    Article Snippet: .. ProteinaseK (NEB) was added to the cell suspension to a final concentration of 75 mg/ml followed by O.N. incubation on the shaking heat block at 55 C 1000 rpm. ..

    Suspension:

    Article Title: Acute multi-level response to defective de novo chromatin assembly in S-phase
    Article Snippet: .. ProteinaseK (NEB) was added to the cell suspension to a final concentration of 75μg/ml followed by O.N. incubation on the shaking heat block at 55 C° 1000 rpm. ..

    Article Title: Acute multi-level response to defective de novo chromatin assembly in S-phase.
    Article Snippet: .. ProteinaseK (NEB) was added to the cell suspension to a final concentration of 75 mg/ml followed by O.N. incubation on the shaking heat block at 55 C 1000 rpm. ..

    Blocking Assay:

    Article Title: Acute multi-level response to defective de novo chromatin assembly in S-phase
    Article Snippet: .. ProteinaseK (NEB) was added to the cell suspension to a final concentration of 75μg/ml followed by O.N. incubation on the shaking heat block at 55 C° 1000 rpm. ..

    Article Title: Acute multi-level response to defective de novo chromatin assembly in S-phase.
    Article Snippet: .. ProteinaseK (NEB) was added to the cell suspension to a final concentration of 75 mg/ml followed by O.N. incubation on the shaking heat block at 55 C 1000 rpm. ..



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